![]() Additional second messenger signaling, e.g. Use cell lines or frozen cells with chemiluminescent or fluorescent assays, and parental cell lines to generate your own GPCR stable cell lines. Gα15 is a promiscuous G protein to help funnel signaling to a common calcium readout, regardless of G protein coupling status. ChemiSCREEN Stable Cell Lines, Parental Cell Lines, and Ready-to-Assay Frozen Cells – Cells based on our proprietary Chem-1 and related host cell backgrounds that contain an endogenous expression of Gα15.Calcium Gq-coupled Cell Lines - Naturally coupled, stable Gq-coupled cell lines and qualified for use with the Calcium No Wash PLUS Assay, Assa圜omplete™ cell culture reagents, and control ligands.Note aequorin-based luminescence assays require addition of a coelenterazine substrate like coelenterazine H.Ĭell Lines and Ready-to-Assay Frozen Cells Coelenterazine H – Utilize a derivative of native coelenterazine that is significantly more sensitive to calcium and provides enhanced performance in aequorin-based luminescent HTS calcium assays.Calcium No Wash PLUS Detection Kit – Rapidly quantify calcium flux with homogeneous, fluorescent dye-based assays qualified for use with all Gq-coupled cell lines.Qualified for cAMP and calcium second messenger assays with results within 24 hours to accelerate your data generation.Ready-to-assay GPCR frozen cells come with plating media and enough cells to run either 96- or 384-well plate assays in full or half plate formats.Tool box capabilities allows generation of your own stable cell linesĬhemiSCREEN™ and ChemiBRITE™ Frozen Cells.Exceptional high signal-to-background ratio with no assay interference with auto-fluorescent compounds.Cell lines modified to flash with added brightness upon GPCR activation.cAMP, and ERK phosphorylation, for most targets Perform orthogonal assay confirmation such as second messenger signaling, e.g. ![]()
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